Electronic Detection and Diagnosis of Complex Regional Pain Syndrome
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Abstract
Complex Regional Pain Syndrome (CRPS) is a chronic disease that is random and abrupt. As a result, an accurate diagnosis and treatment have yet to be identified due to numerous symptoms and causes. Currently, the method of diagnosis of CRPS is the Budapest Criteria. However, this criterion relies on the patient and doctor’s subjective judgments. Therefore, we propose to develop a diagnostic method for CRPS, integrating reliable and objective analysis of common patterns that occur in patients’ genetic and neuronal structures. Utilizing a zinc oxide biosensor, it was shown the patients’ blood samples with high concentrations of MMP-9, and neuroinflammation occurs as the immune system is impaired. By analyzing blood samples, we quantified the number of CD4+ T cells that penetrate nerves, with direct human CD4+ T cell counting. We compared the structure of neuron cells and identified damage in CRPS patients that were located primarily on dendrites. The significance of using DNA detection and neuron biochips will dramatically improve the diagnosis and treatment of CRPS.
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References
Smart, K. M., Ferraro, M. C., Wand, B. M., & O’Connell, N. E. (n.d.-b). Physiotherapy for pain and disability in adults with complex regional pain syndrome (CRPS) types I and II. Cochrane Library, 2022(8). https://doi.org/10.1002/14651858.cd010853.pub3
Harden, R. N., McCabe, C. S., Goebel, A., Massey, M., Suvar, T., Grieve, S., & Bruehl, S. (n.d.). Complex Regional Pain Syndrome: Practical Diagnostic and Treatment Guidelines, 5th edition. Pain Medicine, 23(Supplement_1), S1–S53. https://doi.org/10.1093/pm/pnac046
Zhu, H., Wen, B., Xu, L., & Huang, Y. (n.d.). Identification of Potential Inflammation-Related Genes and Key Pathways Associated with Complex Regional Pain Syndrome. Biomolecules, 13(5), 772. https://doi.org/10.3390/biom13050772
Janicki, P. K., Alexander, G. M., Eckert, J., Postula, M., & Schwartzman, R. J. (n.d.). Analysis of Common Single Nucleotide Polymorphisms in Complex Regional Pain Syndrome: Genome Wide Association Study Approach and Pooled DNA Strategy. Pain Medicine, 17(12), 2344–2352. https://doi.org/10.1093/pm/pnw133
Kang, H., Noh, S., Hwang, J., Lee, J., & Gang, S. (2022). Genetic role in the pathogenesis of complex regional pain syndrome: a review. www.jbtr.or.kr. https://doi.org/10.12729/jbtr.2022.23.4.109
Escolano-Lozano, F., Gries, E., Schlereth, T., Dimova, V., Baka, P., Vlckova, E., König, S., & Birklein, F. (n.d.-b). Local and Systemic Expression Pattern of MMP-2 and MMP-9 in Complex Regional Pain Syndrome. the Journal of Pain/Journal of Pain, 22(10), 1294–1302. https://doi.org/10.1016/j.jpain.2021.04.002
Campuzano, S., Yáñez-Sedeño, P., & Pingarrón, J. M. (n.d.). Reagentless and reusable electrochemical affinity biosensors for near real-time and/or continuous operation. Advances and prospects. Current Opinion in Electrochemistry, 16, 35–41. https://doi.org/10.1016/j.coelec.2019.03.006
Monošík, R., Stred’anský, M., & Šturdík, E. (n.d.). Application of Electrochemical Biosensors in Clinical Diagnosis. Journal of Clinical Laboratory Analysis, 26(1), 22–34. https://doi.org/10.1002/jcla.20500
Shabani, E., Abdekhodaie, M. J., Mousavi, S. A., & Taghipour, F. (n.d.-b). ZnO nanoparticle/nanorod-based label-free electrochemical immunoassay for rapid detection of MMP-9 biomarker. Biochemical Engineering Journal, 164, 107772. https://doi.org/10.1016/j.bej.2020.107772
Sher, M., & Asghar, W. (n.d.). Development of a multiplex fully automated assay for rapid quantification of CD4+ T cells from whole blood. Biosensors & Bioelectronics/Biosensors & Bioelectronics (Online), 142, 111490. https://doi.org/10.1016/j.bios.2019.111490
Development of a neuron culture biochip. . . Joint research team of Professor Nam Yoon-ki of KAIST Department of Bio and Brain Engineering and Professor Seon Woong of Korea University College of Medicine | News > Bio News > Trends | BRIC. (n.d.-b). BRIC. https://www.ibric.org/bric/trend/bio-news.do?mode=view&articleNo=8776498&title=%EC%8B%A0%EA%B2%BD%EC%84%B8%ED%8F%AC+%EB%B0%B0%EC%96%91+%EB%B0%94%EC%9D%B4%EC%98%A4%EC%B9%A9+%EA%B0%9C%EB%B0%9C..KAIST+%EB%B0%94%EC%9D%B4%EC%98%A4%EB%B0%8F%EB%87%8C%EA%B3%B5%ED%95%99%EA%B3%BC+%EB%82%A8%EC%9C%A4%EA%B8%B0+%EA%B5%90%EC%88%98%EC%99%80+%EA%B3%A0%EB%A0%A4%EB%8C%80+%EC%9D%98%EB%8C%80+%EC%84%A0%EC%9B%85+%EA%B5%90%EC%88%98+%EA%B3%B5%EB%8F%99%EC%97%B0%EA%B5%AC%EC%A7%84#!/list
Azizipour, N., Avazpour, R., Rosenzweig, D. H., Sawan, M., & Ajji, A. (n.d.-b). Evolution of Biochip Technology: A Review from Lab-on-a-Chip to Organ-on-a-Chip. Micromachines, 11(6), 599. https://doi.org/10.3390/mi11060599
Hong, N., & Nam, Y. (n.d.). Neurons-on-a-Chip: In Vitro NeuroTools. Molecules and Cells/Molecules and Cells, 45(2), 76–83. https://doi.org/10.14348/molcells.2022.2023